Next-Gen Genotyping (NGG)

Plant Science

Applications in AgBio, plant biology and biofuel production enable flexible exploration of plant genetics and relevance in commercial markets

Plant Science

From optimizing traits in commercial agricultural crops to understanding plant biology, genetics and genetic testing play an integral role in characterizing key regulatory elements. The genetic testing options for plant science have traditionally been extremely limited in scope and prohibitively expensive, compromising the ability for routine or en mass genotyping of plants. As such, a new technology is desperately needed to allow broad genotyping of low density markers for a significantly reduced cost.

Addressing genetic testing needs for plant research, the NGG has broad application in AgBio and other plant research projects. The assay offers low-cost, high value testing for traits such as yield, environmental resilience and nutritional value for any plant species of interest, including tetraploid species. Eureka Genomics is developing a commercial panel for comprehensive screening of barley in conjunction with the James Hutton Institute and will be broadening the scope of the NGG portfolio for plant science.

Technology

Eureka Genomics’ next-gen genotyping (NGG) provide an improved and economical alternative to traditional technologies for profiling hundreds of SNPs (or other genetic markers) in hundreds to thousands of samples using Next Generation Sequencing (NGS). The assay can be broadly applied to the detection of SNPs, InDels, CNV and methylation and is compatible with DNA or RNA from virtually any organism, even when genome information may be incomplete. The NGG provides rapid, accurate, cost-effective answers by combining discriminatory power of hybridization and ligation, focused high throughput sequencing and rigorously designed statistical analysis.

Eureka Genomics offers access to NGG as either standard or custom designed panels and testing can be performed either as a service through our expert scientific team or in your own lab (with technical support from EG to get you started). The assay is sequencing instrument agnostic, so regardless of the platform you have invested resources into or upgrade to in the future the NGG can be adapted to ensure easy assimilation and rapid data generation for your research or clinical goals. And because NGS is used to provide focused information on only the sequences of interest (and not the rest of the genome), the cost per genotype is extremely low.

NGG Benefits

Platform Agnostic
Compatible with your existing sequencing platform rather than requiring investment in expensive, dedicated workflows.
Flexible Design
Interrogate all of your SNPs of interest in a single assay rather coverage than buying limited, fixed panels or paying premiums for custom assay design.
Faster Answers
Query hundreds of SNPs for hundreds to thousands of samples in parallel rather than one sample at a time.
Build Efficiency
Ask multiple biological questions simultaneously in a single sequencing lane to reduce wasted space.
Reduce Cost
Significantly reduce the price per genotype of custom panel development compared to alternative technologies.
Evolve with Eureka
Leverage the future of NGS-based genetic testing with a technology that will easily adapt to your instrumentation upgrades or conversions.

Products and Services

  • NEW! Barley Genotyping Panel
    Being built in collaboration with the James Hutton Institute and available exclusively through Eureka Genomics
  • COMING SOON! YOUR YOUR Plant Genotyping Panel
    Work with Eureka Genomics to design a low-cost custom plant testing assay
  • PIPELINE for Q4 2013: Arabidopsis thaliana Genotyping Panel
    Screen for desired traits or track strains following experimental crossing

Request a quote today!